Professor Stephen Bustin
0845 196 3570
Qualifications & Memberships
- PhD University of Dublin Trinity College
- Fellow of the Society of Biology (FSB)
Outline of careerStephen Bustin BA(Mod) PhD FSB obtained his PhD from Trinity College Dublin. Following post-doctoral research into Foot and Mouth Disease Virus at the Animal Virus Research laboratory in Pirbright and molecular technologies at Corporate Research, Amersham International, he became a Senior Research fellow (1989) and a Senior Lecturer (1995) at the London Hospital Medical College.
Following the merger with St Bartholomew's Medical College and Queen Mary University of London he was promoted to Reader in Molecular Medicine in 2002, followed by the award of a personal chair as Professor of Molecular Science in 2004 at Barts and the London School of Medicine and Dentistry.
He is also a visiting Professor of Molecular Biology at the University of Middlesex.
Research InterestDuring his scientific career of nearly thirty years his main area of interest has been the investigation of the biology of cancers with a particular emphasis on the role of endocrine factors on tumourigenesis. Many of his publications describe important advances in colorectal and breast cancers and a continuing theme of his research has been the translation of novel ideas into clinical practice.
A second area of interest focuses on nucleic acid-based assays targeting common human pathogens such as Clostridium difficile and Aspergillus. He is a world-renowned expert on real-time PCR, the most widespread technology used in life science, molecular medicine and diagnostics and has published numerous peer-reviewed papers that describe and use this technology.
He wrote and edited the "A-Z of Quantitative PCR" (2004), universally acknowledged as the "qPCR Bible" as well as "The PCR Revolution" (2011). He led the international consortium that drew up the MIQE guidelines (2009) and is in constant demand as a speaker and teacher at international qPCR meetings and workshops. At the 2007 Autism trial at the Office of Special Masters of the U.S. Court of Federal Claims he was an expert witness for the Department of Justice in the Cedillo v. Secretary of Health and Human Services (case #98-916V).
Professor Bustin has extensive editorial involvements as Editor-in-Chief, Gene Expression, International Journal of Molecular Sciences, Section Editor, Gene Expression, BMC Molecular Biology, member of editorial board of Gene Regulation and Systems Biology (Libertas Academica) and member of editorial board of Biomarkers in Medicine (Future Science Group). He is also a member of the organising committee of the premier European PCR congress, held bi-annually in Munich and the US PCR congress, held annually in San Francisco.
Selected PublicationsJohnson G, Nolan T, Bustin SA. Real-time quantitative PCR, pathogen detection and MIQE. Methods Mol Biol. 2013 PMID: 23104279.
Bustin SA, Murphy J. RNA biomarkers in colorectal cancer. Methods. 2012 PMID: 23079397.
Johnson GL, Bibby DF, Wong S, Agrawal SG, Bustin SA. A MIQE-compliant real-time PCR assay for Aspergillus detection. PLoS One. 2012 PMID: 22808087
Bustin S, Bergkvist A, Nolan T. In silico tools for qPCR assay design and data analysis. Methods Mol Biol. 2011 PMID: 21780004.
Bustin SA. Developments in real-time PCR research and molecular diagnostics. Expert Rev Mol Diagn. 2010 PMID: 20843195.
McCarthy K, Laban C, Bustin SA, Ogunkolade W, Khalaf S, Carpenter R, Jenkins PJ. Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase, and vitamin D receptor mRNA in normal and malignant breast tissue. Anticancer Res. 2009 PMID: 19331145.
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R,Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009 PMID: 19246619.
Banerjea A, Hands RE, Powar MP, Bustin SA, Dorudi S. Microsatellite and chromosomal stable colorectal cancers demonstrate poor immunogenicity and early disease recurrence. Colorectal Dis. 2009 PMID: 18637931.
Sindelka R, Jonák J, Hands R, Bustin SA, Kubista M. Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte. Nucleic Acids Res. 2008 PMID: 18039714.
Nolan T, Hands RE, Bustin SA. Quantification of mRNA using real-time RT-PCR. Nat Protoc. 2006 PMID: 17406449.
Find out more about Stephen's publications